Carrara, M., L. Cima, R. Cerini and M. Dalle Carbonare. A New In Vitro Model for Predicting the Toxicity of Cosmetic Products. ATLA. 1992. 20: 138-143.

liquid petrolatum - 8012-95-1; sodium dodecyl sulfate - 151-21-3; isopropyl myristate - 110-27-0; cetearyl alcohol - 67762-27-0; petrolatum - 8009-03-8; oleyl alcohol - 593-47-5; lanolin - 8006-54-0; sorbitan sesquioleate - 8007-43-0; glycerol - 56-81-5; dimethicone - 9006-65-9; acrylamide/sodium acrylamide copolymer

A method has been developed whereby cosmetic products which are not soluble in water or in alcohol can be brought into contact with cell cultures by being placed in a cell culture insert, which is then placed in the cell culture well. Preliminary experiments were carried out with L929 cells and cytotoxicity was evaluated by measuring neutral red uptake and the total protein content of treated cultured cells. Encouraging results were obtained in comparisons of three cosmetic emulsions and of one emulsion containing a range of concentrations of two preservatives, Kathon CG and Bronopol.