Benassi, L., G. Bertazzoni, C. Magnoni, A. Di Nardo, E. Euclidi and S. Seidenari. Sodium Lauryl Sulphate Causes Interleukin-1a Release from Normal Human keratinocytes: Dose-Response and Time-Course Curves. ATLA 1999. 27: 343.

Sodium lauryl sulfate – 00151-21-3

Interleukin-1a is involved in the biological response to irritants as one of the most important inflammatory mediators. The aim of the present study was to evaluate IL-1a production after exposure of cultured human keratinocytes to sodium lauryl sulphate (SLS), representing a model which enables the assessment of the in vitro effects of SLS on epidermal cells independently from the skin immune system and barrier alterations. Normal human keratinocytes from plastic surgery were grown in serum free medium. At subconfluency, cells were treated with SLS doses ranging from 0.00001% to 0.005%, and after exposure for one hour, the medium was changed. At different time-points, the supernatant was collected for ELISA, and cells were harvested for Western blot analysis of pro-IL-1a and IL-1a. Extracellular secretion of I-1a from keratinocytes was increased in a dose-dependent manner following SLS treatment. The release of IL-1a starts 30 minutes after exposure to SLS (effective dose 50%) reaching a maximum at 3 hours. Western blot analysis showed a down regulation of pro-IL-1a levels, while IL-1a levels remained unchanged.