Inman, A.O. and N.A. Monteiro-Riviere. An Autoradiography Study To Evaluate the Distribution of Bis(2-Chloroethyl)Sulfide in the Isolated Perfused Porcine Skin Flap. The Toxicologist 1995. 15:324.

sulfur mustard - 00505-60-2

Bis(2-chloroethyl)sulfide (sulfur mustard, HD), a potent cutaneous vesicant and bifunctional alkylating agent, causes macroscopic and microscopic vesicles in the isolated perfused porcine skin flap (IPPSF). Six IPPSFs were topically dosed with 300 ul of 10.0 mg/ml ¹⁴C-HD in ethanol (9.36 uCi) and perfused for 8 hrs. Skin samples were frozen and autoradiography (sandwich technique) performed on 4 um cryosections to minimize diffusion of the ¹⁴C-HD. The emulsion was exposed to sections containing ¹⁴C-HD within the upper epidermis which decreased with dermal depth. Specific binding was present within the capillaries of the dermis. Morphometric analysis of the autoradiographs demonstrated that ¹⁴C-HD occurred predominantly within a tissue depth of 80 um, which incorporates the entire epidermis and the superficial dermis.