Pittermann, W., B. Jackwerth, and M. Schmitt. The Isolated Perfused Bovine Udder Skin Model: A New In Vitro Model for the Assessment of Skin Penetration and Irritation. In Vitro Toxicol. 1997. 10(1): 17-21.

sodium lauryl sulfate - 00151-21-3;

The isolated perfused bovine udder skin (BUS) model was developed for studies concerning percutaneous absorption of pharmaceutical substances (Kietzmann et al., 1993). Additionally this in vitro method provides new possibilities for the assessment of the skin irritation potential. The comparison of the prostaglandin E₂-concentrations and MTT values of untreated control sites obtained simultaneously to treated skin sites showed the unimpaired vitality during the perfusion period of 20 individual bovine udder studies for assessment of skin irritation. Furthermore the results reveal no seasonal influences on the suitability of the organs perfused.

Out of a number of tests concerning body care ingredients and formulations the results of two surfactants widely used were selected. Sodium lauryl sulfate (SLS) and alkyl polyglycoside (APG) were applied on the udder skin with low concentration of 3% and 10% active substance, respectively, under occlusive conditions for 1 h and 5 h. The biological effects in the epidermal and dermal layers were characterized by means of prostaglandin E₂ assay and methyl tetrazolium salt dye conversion. The two surfactants could be clearly differentiated. The skin mildness of APG concerning cytotoxicity and synthesis of eicosanoids (e.g. irritancy after exposure up to 5 h) was proven in conformity with human studies.