Sanders, C., T. D. Swedlung, R. J. Stephens, and P. M. Silber. Evaluation of Six In Vitro Toxicity Assays: Comparison with In Vivo Ocular and Dermal Irritation Potential of Prototype Cosmetic Formulations. The Toxicologist. February 1991. 11(1): 181 (Abstract #1085).

There has been an increasing interest in the potential of in vitro toxicity assays to serve as accurate and inexpensive non-animal predictive models of in vivo ocular and dermal irritation potential for many types of products and raw materials. In this study sixteen different cosmetic formulations including oil-in-water emulsion systems, hydroalcoholic products, and cleansers were evaluated using the EYTEX assay, the chorioallantoic membrane vascular assay (CAMVA), Microtox, SKINTEX, the Tetrahymena motility assay and the TESTSKIN Living Dermal Equivalent (LDE) cytotoxicity assay. These in vitro analyses were compared to historical in vivo ocular and dermal irritation data for these same test materials. Rank-order analyses and regression modeling indicate that CAMVA, the Tetrahymena motility assay, the LDE cytotoxicity assay, and the Microtox assay all exhibited good correlation with corresponding in vivo data. In contrast, neither the EYTEX nor the SKINTEX assays exhibited good correlation with the in vivo data.